What is methylation ratio and how to I improve it to improve on my breast cancer risk?
Q: My results for your Estronex Lab Test for breast cancer risk showed that several of the undesirable estrogen metabolites were high and that my Methylation ratios should be improved. What is methylation? I already take a multi-vitamin every day that contains 400 mcg of folate and 300 mcg of Vitamin B12. Do I need to take more?
A: Your lab test shows that your undesirable estrogen metabolites named 4-Hydroxyestrone (4-OHE1), and 2-OHE1 / 2-OMeE1 can be improved upon by improving on your methylation ratios. This can be done by adding cofactors (Vitamin B12 or folate) and methyl donors (such as betaine or DMG). I recommend you test functional need for vitamin B12 or folate.
Methylation is a biochemical reaction in the body where a nutrient donates a methyl group (CH3) to another substance. Folate and B12 (in the form of methylcobalamin) both do this along with SAMe, Betaine & DMG (& B6 is also helpful). Here is an example of what this type of reaction looks like:
Since you are on bioidentical hormones and are are at menopausal age, you’ll need to reference the 3rd column, that says “Post-Menopausal Females (on hormone therapy)”. Since you are on hormonal therapy, the 4-Hydroxyestrone (4-OHE1) does come out to be normal for post-menopausal women on hormones. However, your methylation ratio which is calculated by 2-Hydroxyestrone (2-OHE1) / 2-Methoxyestrone (2-OMeE1) is 7.4, is higher than is should be.
In phase II detoxification (this occurs in the liver), the estrogen metabolites are conjugated with compounds that allow excretion by the body. Adequate methylation is very important to properly clear estrogens. 2-Hydroxyestrone and 4-hydroxyestrone are methylated in Phase II to produce 2-methoxyestrone (2-OMeE1) and 4-methoxyestrone (4-OMeE1). 2-OMeE1 has anticancer effects and studies show that it is lower in breast cancer patients than controls. It has been referred to as a “good” estrogen. 4-OMeE1 is a non-carcinogenic metabolite of 4-OHE1.
Your 2/16 Hydroxyestrogen Ratio is where is should be which is good. So main thing to focus on is to improve methylation as stated in your results. It is good you are taking a multivitamin with folate and B12. You may want to look at what you are taking to determine if it has methylcobalamin or a different kind of B12. If it is different, then it would be helpful for you to switch to a multi which offers methylcobalamin.
Reducing stress is important for this enzyme to work towards estrogen metabolism. It would be a good idea to add other methylation factors to help improve this ratio. A good one to add would be this:
Methyl Guard by Thorne – twice daily with meals in addition to your multivitamin.
I would suggest adding this for 3 months – 6 months then retesting with this Estronex Test again to see if this has improved.
If you would like to schedule a consult with me to go over your test results by phone, please go to the Consultation page and select the type of consult you would like.